TaUAM3, a UDP‐Ara mutases protein, positively regulates wheat resistance to the stripe rust fungus

Plant cell walls are composed of polysaccharides such as cellulose, hemicelluloses, and pectins, whose location function differ depending on plant type. UDP-Ara mutases (UAMs) have been reported to play important roles in development response various stresses (abiotic biotic). However, little work has UAM wheat. In this study, we dissected the role family member, UAM3, during interaction between wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), treatment with salicylic acid (SA). RNA interference (RNAi)-based stable silencing TaUAM3 resulted decreased resistance Pst fungus. addition, CRISPR-mediated genome editing (GE) enhanced susceptibility or compromised disease accompanied by increased fungal growth H2O2 production tissues. Moreover, transcript levels pathogenesis-related (PR) genes reactive oxygen species (ROS)-generating were down-regulated both RNAi-silenced CRISPR-edited plants, while ROS-scavenging gene, TaCAT3, was up-regulated. Therefore, positively regulates Pst.